Four component structure prediction

In this recipe, I will reproduce the prediction of the stirrup structures from this paper. Using minimal models of ditopic and tetratopic building blocks, we generated all possible homoleptic and heteroleptic graphs and building block configurations with 2:2:1:1, 2:2:0:2 or 2:2:2:0 stoichiometries and compute their relative stability, aiming for the most stable structure. Note that the forcefield is not modified, but defined from atomistic building blocks.

../_images/recipe_5.png

We will start by defining our definer_dict for the constant terms, and the associated bead library.

# Define a database, and a prefix for naming structure, forcefield and
# output files.
database_path = data_dir / "test.db"

# Define a definer dictionary.
# These are constants, while different systems can override these
# parameters.
cg_scale = 2
constant_definer_dict = {
    # Bonds.
    "mb": ("bond", 1.0, 1e5),
    # Angles.
    "aca": ("angle", 180, 1e2),
    "ede": ("angle", 180, 1e2),
    "mba": ("angle", 180, 1e2),
    "mbe": ("angle", 180, 1e2),
    # Nonbondeds.
    "m": ("nb", 10.0, 1.0),
    "d": ("nb", 10.0, 1.0),
    "e": ("nb", 10.0, 1.0),
    "a": ("nb", 10.0, 1.0),
    "b": ("nb", 10.0, 1.0),
    "c": ("nb", 10.0, 1.0),
    "f": ("nb", 10.0, 1.0),
}

# Define beads.
bead_library = cgx.molecular.BeadLibrary.from_bead_types(
    # Type and coordination.
    {"m": 4, "a": 2, "b": 2, "c": 2, "d": 2, "e": 2, "f": 2}
)

Then we can map that to our building block library.

# Define your forcefield alterations as building blocks.
building_block_library = {
    "lin": {
        "precursor": cgx.molecular.TwoC1Arm(
            bead=bead_library.get_from_type("c"),
            abead1=bead_library.get_from_type("a"),
        ),
        "mod_definer_dict": {
            "ba": ("bond", 2.8 / cg_scale, 1e5),
            "ac": ("bond", 1.5 / 2 / cg_scale, 1e5),
            "bac": ("angle", 180, 1e2),
        },
    },
    "mxy": {
        "precursor": cgx.molecular.TwoC1Arm(
            bead=bead_library.get_from_type("d"),
            abead1=bead_library.get_from_type("e"),
        ),
        "mod_definer_dict": {
            "be": ("bond", 7.6 / cg_scale, 1e5),
            "ed": ("bond", 5.0 / 2 / cg_scale, 1e5),
            "bed": ("angle", 90, 1e2),
        },
    },
    "tetra": {
        "precursor": cgx.molecular.FourC1Arm(
            bead=bead_library.get_from_type("m"),
            abead1=bead_library.get_from_type("b"),
        ),
        "mod_definer_dict": {
            "mb": ("bond", 2.0 / cg_scale, 1e5),
            "bmb": ("pyramid", 90, 1e2),
        },
    },
    "corner": {
        "precursor": cgx.molecular.TwoC0Arm(
            bead=bead_library.get_from_type("f"),
        ),
        "mod_definer_dict": {
            "bf": ("bond", 2.0 / cg_scale, 1e5),
            "bfb": ("angle", 90, 1e2),
            "fbm": ("angle", 90, 1e2),
        },
    },
}

And define a series of systems to explore. Here, I only check particular homoleptic and heteroleptic combinations.

# Define systems to predict the structure of.
systems = {
    "mix1_2-2-1-1": {
        "stoichiometry_map": {"tetra": 2, "corner": 2, "lin": 1, "mxy": 1},
        "multipliers": (1,),
        "vdw_cutoff": 2,
    },
    "mix1_2-2-2-0": {
        "stoichiometry_map": {"tetra": 2, "corner": 2, "lin": 2},
        "multipliers": (1,),
        "vdw_cutoff": 2,
    },
    "mix1_2-2-0-2": {
        "stoichiometry_map": {"tetra": 2, "corner": 2, "mxy": 2},
        "multipliers": (1,),
        "vdw_cutoff": 2,
    },
}

Time to iterate!

for system_name, syst_d in systems.items():
    logger.info("doing system: %s", system_name)
    # Merge constant dict with modifications from different systems.
    merged_definer_dicts = cgx.systems_optimisation.merge_definer_dicts(
        original_definer_dict=constant_definer_dict,
        new_definer_dicts=[
            building_block_library[i]["mod_definer_dict"]
            for i in syst_d["stoichiometry_map"]
        ],
    )

    forcefield = cgx.systems_optimisation.get_forcefield_from_dict(
        identifier=f"{system_name}ff",
        prefix=f"{system_name}ff",
        vdw_bond_cutoff=syst_d["vdw_cutoff"],
        present_beads=bead_library.get_present_beads(),
        definer_dict=merged_definer_dicts,
    )

    # Build all the building blocks and pre optimise their structures.
    bb_map = {}
    for prec_name in syst_d["stoichiometry_map"]:
        prec = building_block_library[prec_name]["precursor"]
        if prec_name == "corner":
            bb = prec.get_building_block()
        else:
            bb = cgx.utilities.optimise_ligand(
                molecule=prec.get_building_block(),
                name=f"{system_name}_{prec.get_name()}",
                output_dir=calc_dir,
                forcefield=forcefield,
                platform=None,
            ).clone()
            bb.write(
                str(
                    ligand_dir
                    / f"{system_name}_{prec.get_name()}_optl.mol"
                )
            )
        bb_map[prec_name] = bb

    for multiplier in syst_d["multipliers"]:
        logger.info(
            "doing system: %s, multi: %s", system_name, multiplier
        )

        # Define a connectivity based on a multiplier.
        iterator = cgx.scram.TopologyIterator(
            building_block_counts={
                bb_map[name]: stoich * multiplier
                for name, stoich in syst_d["stoichiometry_map"].items()
            },
        )
        logger.info(
            "graph iteration has %s graphs", iterator.count_graphs()
        )

        possible_bbdicts = iterator.get_configurations()
        logger.info(
            "building block iteration has %s options",
            len(possible_bbdicts),
        )

        logger.info(
            "iterating over %s graphs and bb configurations...",
            iterator.count_graphs() * len(possible_bbdicts),
        )
        run_topology_codes: list[agx.ConfiguredCode] = []
        for bb_config, topology_code in it.product(
            possible_bbdicts,
            iterator.yield_graphs(),
        ):
            configured = agx.ConfiguredCode(topology_code, bb_config)
            if not agx.utilities.is_configured_code_isomorphic(
                test_code=configured,
                run_topology_codes=run_topology_codes,
            ):
                continue

            run_topology_codes.append(configured)

            # Here we apply a multi-initial state, multi-step geometry
            # optimisation algorithm.
            config_name = (
                f"{system_name}_{multiplier}_"
                f"{topology_code.idx}_b{bb_config.idx}"
            )

            # Each conformer is stored here.
            conformer_db_path = calc_dir / f"{config_name}.db"
            optimisation_workflow(
                config_name=config_name,
                conformer_db_path=conformer_db_path,
                topology_code=topology_code,
                iterator=iterator,
                bb_config=bb_config,
                calculation_dir=calc_dir,
                forcefield=forcefield,
            )

            conformer_db = cgx.utilities.AtomliteDatabase(
                conformer_db_path
            )
            min_energy_structure = None
            min_energy = float("inf")
            min_energy_key = None
            for entry in conformer_db.get_entries():
                if entry.properties["energy_per_bb"] < min_energy:
                    min_energy = entry.properties["energy_per_bb"]
                    min_energy_structure = conformer_db.get_molecule(
                        key=entry.key
                    )
                    min_energy_key = entry.key

            # To file.
            min_energy_structure.write(
                str(struct_output / f"{config_name}_optc.mol")
            )

            # To database.
            cgx.utilities.AtomliteDatabase(database_path).add_molecule(
                molecule=min_energy_structure, key=config_name
            )
            properties = {
                "multiplier": multiplier,
                "topology_idx": topology_code.idx,
            }
            cgx.utilities.AtomliteDatabase(database_path).add_properties(
                key=config_name, property_dict=properties
            )

            analyse_cage(
                database_path=database_path,
                name=config_name,
                conformer_db_path=conformer_db_path,
                min_energy_key=min_energy_key,
            )

And now we can plot the most stable structure for each multiplier. graph and building block configuration combination (different stoichiometries are split by the horizontal lines). The data shows that the homoleptic structure: mix1_2-2-0-2_1_0_b5 (with the bent ligand) is the most stable (along side another homoleptic structure with a different graph):

../_images/recipe_5_test.png

While the heteroleptic stirrup (mix1_2-2-1-1_1_0_b8 is also stable, but not as stable. However, the flexibility of the bent ligand and the exchange reaction to form the heteroleptic can explain this outcome.

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